11/12/2024

I measured the salinity of the media again today, and it was HIGHER than yesterday, which is not the result that I was looking for. I learned that Halobacterioum needs oxygen or light to be able to grow, so I am moving them from the refrigerator to out in the open covered in parafilm instead of an air-tight seal. While I continue to measure their salinity consumption rate, I will also be researching what genetics are responsible for their internal salinity regulation/external salinity consumption. Hopefully, I will be able to duplicate those genetics to get the bacteria to process even more salt. I still need to research how to modify the DNA, though.

(Fig 11/12/2024) day 2 of measuring the halobacterium media salinity.

11/11/2024

I have successfully made the media for the Halobacterium Salinarum, and it spent last Thursday and Friday in an incubator at 42C. I did remove it and refrigerate it over the weekend however. I realize that I probably should have measured the salinity before adding the bacteria, but oh well. I measured the salinity today(fig 1.).

(Fig 11/11/2024) The salinity and temperature measurement for the halobacterium media

using a Hoicated eclectic salinity measurer

9/24/2024

This week I have been mostly waiting for my materials to arrive. In the meantime, I have completed my researcher profile, which is viewable at the bottom of the page. I have also started writing the procedure for growing and measuring the salt consumption of Halobacterium, which is available on the Synthetic Biology team page.

9/9/2024

the synthetic biology lab was officially approved by the WISRD board last week. Since then I have started researching and organizing our first experiment. I am uploading all of my notes onto the WISRD slack page under #team-synthetic-biology. We will start by growing Halobacterium salinarum. We will then measure the rate and efficacy(how much salt is left) of the Archaea.

Here is a list of materials I sent to Megan:

• Halobacterium sp. NRC-1- 

• Submersible water heater

• 6 Gallon Tank- ordering 9/9

• Tank Aerator ordering 9/9

• 5.75 kg NaCl- we have this 

• 460 g MgSO4(magnesium sulfate) - Tim has this

• 46 g KCl- WISRD has this

• 69 g NaH2C6H5O7 (Sodium Citrate dihydrate)- order this 

• Oxoid Peptone - order this

• Stir Plate & Bar (or a Stir Stick if not available)- we have this

• 1 L Beaker- we have this

• 1 L Distilled Water- we make this

• 1 L Graduated Cylinder- we have this

• 1 L Bottle- we should get this

• .01 g – 300 g capable Scale- we have this

• Weigh boats and spatulas- we have this

1/20/2023

To-do list:

• Define cosmic ray shower

• How often do cosmic ray showers occur?

• try reading CERN article

• How often should he be receiving them?

• find CRD dimensions and divide by how often showers occur

• read CRD manual

• list our collected events with heights rate of coincidences

• what should make the cut off?

• Make observatory remote

• finish stepper motor set up

• ask Noam for more details

We have our goals now we must work towards them. my goal is the write a paper on our definition of cosmic ray shower by the end of the year.

11/9/2022

Noam has gotten the stepper motors to run. I have started working on a paper about the standard model. Dark Matter Day went well, and my station was a success. starting to work on the next poster presentation on December 7th.

10/11/2022

Noam has picked up the Stepper motor project, which is giving me time to work on analyzing data he is collecting with our cosmic ray detectors. I am using databases of known supernovas to check if the data we collected was special at those times. We are looking for supernovas specifically because capturing cosmic ray showers from supernovas can tell us about the life cycle of stars, which can give us insight into our own stars.

9/27/2022

I’m back! and have been very busy. I have recruited two new members to the cosmic ray group(Noam and Jacob). I have also started an internship for Quarknet out of UCI under Michael Smy and Dr. Pedro Ochoa-Ricoux. In class, I am working on controlling an Arduino using a Raspberry PI so we can interact with the cosmic ray detectors remotely.

4/26/2022

CRD control panel is under cd Downloads>(up arrow until you see the long file name)

CRD coincidence chart for port one looks weird(double peaks)

Port voltages read:

Port 1: 0.739

Port 2: 0.804

Port 3: 0.768

Port 4: 0.763

This reading is normal and hasn’t changed recently. why would the graph change if the voltage is the same?

4/13/2022

In order to find the Radio Telescope raw data I had to use FileZilla to connect to the Pi holding the data (192.168.168.34:5300). Once you have connected, the files will be under

Home >radioastro>reaserch>gr-radio_astro>examples>data

The raw data should be downloadable. Download it to a computer (into a file, or it's a mess) and drop the file into the Google Drive Radio telescope raw data repository and once you are finished downloading, you are good to go!

3/21/2022

Started collecting Data from the radio telescope at 9:05 AM

2/18/2022

To do list:

• cover and seal solar panel

• fix fan to the pelican

• access pelican case for ethernet cables

• complete PVC line for cables

• calibrate cosmic ray detector

• finish water proof boxes

2/16/2022(wed)

First Radio telescope roof test.

conditions: windy, clear skies

Unite: RadioTelescopeN (north)

Pi# 5300

started taking calibration .Hot files at 14:00

first file: 22-02-16T215627.hot

finished gathering .Hot files at 14:18 with 10 .Hot file in total

2/4/2022(fri)

today I committed carpentry and sealed the wood boxes using silicon. I evenly placed the silicone anywhere the wood connected and made sure to fill in all the cracks as I screwed to board into place. the end product was a fully water tight box. (I wasn’t able to insert any pictures due to file incompatibility they are on my google drive email if needed.)

1/24/2022(mon)

how to start the spectrometer calibration –

step 1) once you have set up the radio telescope (Image one) download and open VNC viewer

step 2) connect VNC viewer to the pi by entering the server address (192.168.168.## rest of the number should be labeled on the pi) and entering the user name and passcode (username: RadioAstro and the password is the regular WISRD, ask joe if you don know it)

step 3) open the directory panel and type the following command; cd examples/nsf than type NsfIntegrate100

Step 4) once you have reached the control screen for the spectrometer change the values in the gain 2 to 0, gain to 0 ,and gain 3 to 0 in that order. then turn gain 2 to 14, gain 3 to 14, and gain 1 to 18 in that order

step 5) flip the horn of the radio telescope to face the ground and change the elevation on the spectrometer control screen to -90 (remember to hit enter)

step 6) change the ods:: setting to Hot/Cold and the Rec:: to Average

step 7) the

To be continued…

1/10/2022(mon)

Today I learned the difference between the period and the frequency of a wave, the period is the amount of time it takes for a full wave length to pass a point in space and the frequency is how many pass in one second.

12/8/2021(Thr)

Many things have happened since my last report. I wave been focused on my Vitae as well as continuing to work on the DISPIRA white paper and attending GAVRT sessions. I was also promoted to the position of GAVRT PI(primary investigator) due to me being the only member of GAVRT currently. I am hopping more people will chose to join the GAVRT group and I am doing my best to advertise and make the group sound appealing to attract new participants.

10/25/2021(Mon)

Continuing white paper. I am assigned to the waves section of the white paper and have finished gathering all the needed knowledge to write my paper on a google doc.

10/5/2021(Tue)

I have started coordinating with my team regarding a experiment involving ionized hydrogen as an easy representation of the hydrogen line. I believe that it would make a good white paper and it would also help us understand how people came to find out about the hydrogen line.

9/27/2021(Mon)

continued reading the Eric Trumbauer article and taking cornel notes

9/23/2021(Thr)

Formally switched to Radio telescopes team and started reading and writing notes on the provided articles

9/21/2021(Tue)

First GAVRT session was mostly practice and making sure I know everything necessary to participate in blackhole patrol

9/14/2021(Tue)

Today a joined the Hydroponics team and continued researching GAVRT in preparation for next Tuesday when am scheduled to use it.